Histochemical Characterization of Early Response to Cochliobolus sativus Infection in Selected Barley Genotypes

Accumulation of Transcripts Abundance after Barley Inoculation with Cochliobolus sativus

Spot blotch caused by the hemibiotrophic pathogen Cochliobolus sativus has been the major yield-reducing factor for barley production during the last decade. Monitoring transcriptional reorganization triggered in response to this fungus is an essential first step for the functional analysis of genes involved in the process. To characterize the defense responses initiated by barley resistant and...

Improvement of Xylanase Production by Cochliobolus sativus in Submerged Culture

The xylanase production by a new Cochliobolus sativus Cs5 strain was improved under submerged fermentation. The xylanase was induced by xylan and repressed by glucose, sucrose, maltose, xylose, starch and cellulose. Highest enzyme production (98.25 IU/mL) was recorded when wheat straw (4 % by mass per volume) was used as a carbon source after 120 h of incubation. NaNO3 increased xylanase produc...

Variation in the response of barley cultivars to nirrogen fertilizer

REACTION OF BARLEY GENOTYPES TO Bipolaris sorokiniana

A study on response of 29 barley genotypes resistant to Bipolaris leaf blight (Bipolaris sorokiniana) was conducted to investigate the relationship of the components of resistance of 29 barley genotypes. Data on five components of resistance viz., infection frequency (number of lesions per plant), lesion size (mm), percent leaf area affected by lesion, percent necrotic area on leaf, and disease...

Analysis of genetic diversity among selected grasspea (Lathyrus sativus L.) genotypes using RAPD markers.

Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variability among five selected genotypes of grasspea. Out of 30 random decamer primers tested for the present investigation 20 showed reproducible DNA amplification. A total of 257 loci were amplified of which 159 were polymorphic including 57 genotype-specific unique bands. Amplicons had molecular weights ra...